YMDD mutations and genotypes of hepatitis B virus in northern China

Di Li, Hong Xi Gu, Shu Yun Zhang, Zhao Hua Zhong, Min Zhuang, Toshio Hattori

研究成果: Article

21 引用 (Scopus)

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The objective of this research was to determine the relationship between YMDD mutations and the genotypes of hepatitis B virus (HBV) during lamivudine treatment. HBV genotypes were determined by nested PCR with 6 pairs of HBV genotype-specific primers (A to F) in serum specimens from 142 hepatitis B patients receiving lamivudine antiviral therapy. YMDD mutations were detected by fluorescent hybridization bioprobe PCR and melting curve assay (FH-PCR-MC). Among 142 serum specimens, 13 samples were genotype B (9.2%), 125 samples were genotype C (88%), 4 samples were genotype D (2.8%), and 80 YMDD mutations were found. The YMDD mutation rates were 69.2 and 54.4% in genotype B and genotype C, respectively. There was no significant difference in the YMDD mutation rate between genotypes B and C. Nine genotype B sera with YMDD mutations were found, including 2 YIDD mutations and 7 YVDD (M + V) mutations. Sixty-eight genotype C sera with YMDD mutations were found, including 34 mutations I (M + I) and 17 mutations V (M + V). There was a significant difference in the YMDD mutation types between genotypes B and C. Our results suggested that the YMDD mutation rate was 56.3% in patients treated with lamivudine for 2-4 years. YIDD was the main mutation type. The YMDD mutation rate showed no significant difference between HBV types B and C (P > 0.05), while the YMDD mutation types showed a significant difference between HBV types B and C in Northern China (X2 = 4.6, P < 0.05).

元の言語English
ページ(範囲)42-45
ページ数4
ジャーナルJapanese journal of infectious diseases
59
発行部数1
出版物ステータスPublished - 2006 3 6

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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  • これを引用

    Li, D., Gu, H. X., Zhang, S. Y., Zhong, Z. H., Zhuang, M., & Hattori, T. (2006). YMDD mutations and genotypes of hepatitis B virus in northern China. Japanese journal of infectious diseases, 59(1), 42-45.