Yeast Rrp14p is a nucleolar protein involved in both ribosome biogenesis and cell polarity

Hiroko Yamada, Chihiro Horigome, Takafumi Okada, Chiharu Shirai, Keiko Mizuta

研究成果: Article査読

18 被引用数 (Scopus)

抄録

We previously cloned RRP14/YKL082c, whose product exhibits two-hybrid interaction with Ebp2p, a regulatory factor of assembly of 60S ribosomal subunits. Depletion of Rrp14p results in shortage of 60S ribosomal subunits and retardation of processing from 27S pre-rRNA to 25S rRNA. Furthermore, 35S pre-rRNA synthesis appears to decline in Rrp14p-depleted cells. Rrp14p interacts with regulatory factors of 60S subunit assembly and also with Utp11p and Faf1p, which are regulatory factors required for assembly of 40S ribosomal subunits. We propose that Rrp14p is involved in ribosome synthesis from the beginning of 35S pre-rRNA synthesis to assembly of the 60S ribosomal subunit. Disruption of RRP14 causes an extremely slow growth rate of the cell, a severe defect in ribosome synthesis, and a depolarized localization of cortical actin patches throughout the cell cycle. These results suggest that Rrp14p has dual functions in ribosome synthesis and polarized cell growth. Published by Cold Spring Harbor Laboratory Press.

本文言語English
ページ(範囲)1977-1987
ページ数11
ジャーナルRNA
13
11
DOI
出版ステータスPublished - 2007 11
外部発表はい

ASJC Scopus subject areas

  • 分子生物学

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