Yeast KEX2 gene encodes an endopeptidase homologous to subtilisin-like serine proteases

Kensaku Mizuno, Tomoko Nakamura, Takehiro Ohshima, Shoji Tanaka, Hisayuki Matsuo

研究成果: Article査読

177 被引用数 (Scopus)

抄録

Yeast Saccharomyces cerevisiae KEX2 gene previously isolated, was characterized as the gene encoding a calcium-dependent endopeptidase required for processing of precursors of α-factor and killer toxin. In this study, we report the amino acid sequence of the KEX2 gene product deduced from nucleotide sequencing. Our results indicate that the KEX2 gene contains a 2,442-bp open reading frame encoding a polypeptide of 814 amino acids. The deduced amino acid sequence contains a region extensively homologous to the members of subtilisin-like serine protease family near the N-terminus. A putative membrane-spanning domain near the C-terminus was also detected. These facts indicate that the KEX2-encoded protein may function as a membrane-bound, subtilisin-like serine protease.

本文言語English
ページ(範囲)246-254
ページ数9
ジャーナルBiochemical and biophysical research communications
156
1
DOI
出版ステータスPublished - 1988 10月 14
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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