X-ray observations of single bio-supramolecular photochirogenesis

Jaewon Chang, Masaki Nishijima, Hiroshi Sekiguchi, Kohei Ichiyanagi, Masahiro Kuramochi, Yoshihisa Inoue, Yuji C. Sasaki

研究成果: Article査読

3 被引用数 (Scopus)


The binding and photochirogenic behaviour of 2-anthracenecarboxylate (AC) with human serum albumin (HSA) have hitherto been investigated and comprehended as time-averaged statistical events by spectroscopic examinations and product analyses. In this study, we employed a diffracted X-ray tracking (DXT) technique to visualize the single-molecular dynamics of free and AC-loaded HSA (AC:HSA = 0, 1, 5 and 10), as well as the AC-HSA complex under photoirradiation, all of which were tethered to gold nanocrystals and hence traceable in real time by DXT. This enabled us to draw a more dynamic picture of the bio-supramolecular photochirogenesis at a single-molecule resolution, detailing the softening and flexibility enhancement of HSA upon binding of ACs to its inter-subdomain IIA-IIB site and the dynamic extrusion of AC dimers produced upon photoirradiation.

ジャーナルBiophysical Chemistry
出版ステータスPublished - 2018 11月

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 有機化学


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