TY - JOUR
T1 - X-ray observations of single bio-supramolecular photochirogenesis
AU - Chang, Jaewon
AU - Nishijima, Masaki
AU - Sekiguchi, Hiroshi
AU - Ichiyanagi, Kohei
AU - Kuramochi, Masahiro
AU - Inoue, Yoshihisa
AU - Sasaki, Yuji C.
N1 - Funding Information:
We are grateful to prof. Tadashi Mori of The Osaka University , Graduate School of Engineering and Prof. Haruo Kozono of Tokyo University of Science, Graduate School of Biological Science for crucial logistical help. This research was supported by a Grant-in-Aid for Scientific Research (A) , (Grant Nos. 22241032 and 21245011 ) from the Ministry of Education, Culture, Sports, Science and Technology , Japan in 2010–2012 and was performed with the approval of the SPring-8 (Proposal Nos. 2011B1299 , 2012A1396 , 2012A1399 , and 2012B1377 ) and Photon Factory Advisory Committee (Proposal No. 2009G510 , 2009G644 , 2011G608 , 2013G622 , and 2015G589 ).
Funding Information:
We are grateful to prof. Tadashi Mori of The Osaka University, Graduate School of Engineering and Prof. Haruo Kozono of Tokyo University of Science, Graduate School of Biological Science for crucial logistical help. This research was supported by a Grant-in-Aid for Scientific Research (A), (Grant Nos. 22241032 and 21245011) from the Ministry of Education, Culture, Sports, Science and Technology, Japan in 2010–2012 and was performed with the approval of the SPring-8 (Proposal Nos. 2011B1299, 2012A1396, 2012A1399, and 2012B1377) and Photon Factory Advisory Committee (Proposal No. 2009G510, 2009G644, 2011G608, 2013G622, and 2015G589).
Publisher Copyright:
© 2018
PY - 2018/11
Y1 - 2018/11
N2 - The binding and photochirogenic behaviour of 2-anthracenecarboxylate (AC) with human serum albumin (HSA) have hitherto been investigated and comprehended as time-averaged statistical events by spectroscopic examinations and product analyses. In this study, we employed a diffracted X-ray tracking (DXT) technique to visualize the single-molecular dynamics of free and AC-loaded HSA (AC:HSA = 0, 1, 5 and 10), as well as the AC-HSA complex under photoirradiation, all of which were tethered to gold nanocrystals and hence traceable in real time by DXT. This enabled us to draw a more dynamic picture of the bio-supramolecular photochirogenesis at a single-molecule resolution, detailing the softening and flexibility enhancement of HSA upon binding of ACs to its inter-subdomain IIA-IIB site and the dynamic extrusion of AC dimers produced upon photoirradiation.
AB - The binding and photochirogenic behaviour of 2-anthracenecarboxylate (AC) with human serum albumin (HSA) have hitherto been investigated and comprehended as time-averaged statistical events by spectroscopic examinations and product analyses. In this study, we employed a diffracted X-ray tracking (DXT) technique to visualize the single-molecular dynamics of free and AC-loaded HSA (AC:HSA = 0, 1, 5 and 10), as well as the AC-HSA complex under photoirradiation, all of which were tethered to gold nanocrystals and hence traceable in real time by DXT. This enabled us to draw a more dynamic picture of the bio-supramolecular photochirogenesis at a single-molecule resolution, detailing the softening and flexibility enhancement of HSA upon binding of ACs to its inter-subdomain IIA-IIB site and the dynamic extrusion of AC dimers produced upon photoirradiation.
KW - Visualization of high speed photoreaction fast and high resolution measurements single molecule measurements photochirogenesis asymmetric catalyst
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U2 - 10.1016/j.bpc.2018.07.003
DO - 10.1016/j.bpc.2018.07.003
M3 - Article
C2 - 30153504
AN - SCOPUS:85052096901
VL - 242
SP - 1
EP - 5
JO - Biophysical Chemistry
JF - Biophysical Chemistry
SN - 0301-4622
ER -