X-ray-induced mutations in Escherichia coli K-12 strains with altered DNA polymerase I activities

Yuki Nagata, Masakado Kawata, Jun Ichiro Komura, Tetsuya Ono, Kazuo Yamamoto

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Spectra of ionizing radiation mutagenesis were determined by sequencing X-ray-induced endogenous tonB gene mutations in Escherichia coli polA strains. We used two polA alleles, the polA1 mutation, defective for Klenow domain, and the polA107 mutation, defective for flap domain. We demonstrated that irradiation of 75 and 50 Gy X-rays could induce 3.8- and 2.6-fold more of tonB mutation in polA1 and polA107 strains, respectively, than spontaneous level. The radiation induced spectrum of 51 tonB mutations in polA1 and 51 in polA107 indicated that minus frameshift, A:T → T:A transversion and G:C → T:A transversion were the types of mutations increased. Previously, we have reported essentially the same X-ray-induced tonB mutation spectra in the wild-type strain. These results indicate that (1) X-rays can induce minus frameshift, A:T → T:A transversion and G:C → T:A transversion in E. coli and (2) presence or absence of polymerase I (PolI) of E. coli does not have any effects on the process of X-ray mutagenesis.

本文言語English
ページ(範囲)93-103
ページ数11
ジャーナルMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
528
1-2
DOI
出版ステータスPublished - 2003 7 25

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学
  • 健康、毒物学および変異誘発

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