WGA lectin binding sites of the apical surface of corti epithelium: Enhancement by back-scattered electron imaging in guinea-pig inner ear

Yukio Katori, Akira Tonosaki, Tomonori Takasaka

研究成果: Review article査読

6 被引用数 (Scopus)

抄録

The apical surface of the guinea-pig organ of Corti was examined with SEM after WGA-lectin histochemistry which has a binding specificity for sialic acid and N-acetyl-glucosamine. The contrast of colloidal gold markers were particularly enhanced by back-scattered electron imaging. WGA lectin showed remarkable preference for the microvilli as well as flat surface areas in the supporting cells. Stereociliary surfaces and intersteriociliary connections of the sensory hair cell were strongly labelled, whilst only rarely the markers were found in the flat areas of the apical surface of the hair cell. The connections, which are known to be involved in the initiation of the mechanoelectrical transduction, should consist of sialic acid and/or N-acetyl-glucosamine.

本文言語English
ページ(範囲)207-212
ページ数6
ジャーナルJournal of Electron Microscopy
45
3
DOI
出版ステータスPublished - 1996

ASJC Scopus subject areas

  • 器械工学

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