W-heterochromatin of chicken; its unusual DNA components, late replication, and chromatin structure

Noriyuki Suka, Yoshinobu Shinohara, Yasushi Saitoh, Hisato Saitoh, Kohei Ohtomo, Masahiko Harata, Edward Shpigelman, Shigeki Mizuno

研究成果: Article査読

32 被引用数 (Scopus)

抄録

About 65% of DNA in the chicken W chromosome has been shown to consist of XhoI and EcoRI family repetitive sequences. These sequences showed remarkable delay in the electrophoretic mobility at low temperature on a polyacrylamide gel. Three dimensional structures of the 0.7-kb XhoI and the 1.2-kb EcoRI family repeating units were estimated to be irregular solenoids using a computer program based on wedge angles of all the 16 dinucleotide steps. Fluorescence in situ hybridization demonstrated that these two family sequences were localized in a major heterochromatic body in an interphase nucleus. Incorporation of bromodeoxyuridine into the W chromosome in the synchronous culture of MSB-1 cells occurred about 1 h later than the peak of S phase. The chromatin structure formed along XhoI and EcoRI family sequences was suggested to be different from the total chromatin or chromatin containing the β-actin gene sequence in that the linker DNA lengths of the former were significantly longer. Fractionation of the HaeIII-digested MSB-1 nuclei yielded a chromatin fraction in which XhoI family sequences were partially enriched. Several DNA-binding proteins showing higher affinity for the XhoI family sequence were present in this fraction.

本文言語English
ページ(範囲)93-105
ページ数13
ジャーナルGenetica
88
2-3
DOI
出版ステータスPublished - 1993 6 1

ASJC Scopus subject areas

  • 動物科学および動物学
  • 遺伝学
  • 植物科学
  • 昆虫科学

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