Voltage‐activated calcium currents in presynaptic nerve terminals of the chicken ciliary ganglion.

H. Yawo

    研究成果: Article査読

    19 被引用数 (Scopus)

    抄録

    1. Calcium currents (ICa) were recorded from presynaptic calyces of ciliary ganglia of the chick embryo under whole‐cell voltage clamp. 2. Only high‐threshold ICa was recorded without any evidence for the presence of low‐threshold Ca2+ channels. 3. High‐threshold (high‐voltage‐activated, HVA) ICa could be classified into non‐inactivating (HVAn) and inactivating (HVAi) components. The mean inactivation time constant of the HVAi component was 213 ms (at 0 mV). The threshold for activation by depolarizing pulses was more negative for the HVAn component than for the HVAi component. The HVAi component was inactivated by 19% at a holding potential of ‐60 mV, while the HVAn component was little affected under this condition. 4. The activation of HVAn component was faster than that of the HVAi component. 5. Both the HVAn and HVAi components were blocked by Cd2+ (50 microM) and La3+ (1 microM). Both components were only slightly affected by Ni2+ (100 microM). The order of potency in blocking was La3+ greater than Cd2+ greater than Ni2+ for both components. Both the HVAi and HVAn components were irreversibly blocked by omega‐conotoxin GVIA(omega‐CgTX, 10 microM). 6. The two components could pharmacologically be distinguished by selective blockade of the HVAn component with nifedipine (2 microM) and D600 (100‐250 microM). 7. HVAn and HVAi components are suggested to represent two different subpopulations of Ca2+ channels. The HVAn subpopulation may be responsible for persistent Ca2+ influx during subthreshold depolarization of the nerve terminal.

    本文言語English
    ページ(範囲)199-213
    ページ数15
    ジャーナルThe Journal of Physiology
    428
    1
    DOI
    出版ステータスPublished - 1990 9 1

    ASJC Scopus subject areas

    • 生理学

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