Visualizing the spatiotemporal dynamics of DNA damage in budding yeast

Chihiro Horigome, Vincent Dion, Andrew Seeber, Lutz R. Gehlen, Susan M. Gasser

研究成果: Article査読

9 被引用数 (Scopus)

抄録

Fluorescence microscopy has enabled the analysis of both the spatial distribution of DNA damage and its dynamics during the DNA damage response (DDR). Three microscopic techniques can be used to study the spatiotemporal dynamics of DNA damage. In the first part we describe how we determine the position of DNA double-strand breaks (DSBs) relative to the nuclear envelope. The second part describes how to quantify the co-localization of DNA DSBs with nuclear pore clusters, or other nuclear subcompartments. The final protocols describe methods for the quantification of locus mobility over time.

本文言語English
ページ(範囲)77-96
ページ数20
ジャーナルMethods in Molecular Biology
1292
DOI
出版ステータスPublished - 2015
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

フィンガープリント

「Visualizing the spatiotemporal dynamics of DNA damage in budding yeast」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル