Visualization of tumor necrosis factor-α distributions within pressure ulcer tissue using the wound blotting method: A case report and discussion

Aya Kitamura, Gojiro Nakagami, Mikako Yoshida, Hiroshi Noguchi, Yoshimi Nishijima, Takeo Minematsu, Ayumi Naito, Jun Sugawara, Hiroki Shibayama, Kazuo Takahashi, Amiko Hakuta, Junichi Umemoto, Nao Terada, Ryo Segawa, Taketoshi Mori, Hiromi Sanada

研究成果: Review article査読

2 被引用数 (Scopus)

抄録

Wound blotting can be used to visualize the protein distribution on a wound bed through protein collection by attaching a nitrocellulose membrane to the wound surface. This study checked for consistency between the protein distributions determined by wound blotting and those determined by removal of the tissue. A patient who was planning to undergo surgical debridement of an ulcer in the sacral region that was caused by lying down for a long period after a cerebral hemorrhage was recruited in Fujisawa City Hospital, Kanagawa, Japan. Wound blotting was performed just prior to surgical debridement and the debrided tissue embedded in paraffin. The ulcer, which had a 2.9 cm major axis, was divided into 20 areas approximately 0.35 cm2 each, and the consistency of tumor necrosis factor-α positivity between the wound blotting samples and tissue sections was examined in each area. The sensitivity and specificity of wound blotting were 89% and 82%, respectively. This wound blotting method noninvasively revealed the protein distributions within the wound tissue.

本文言語English
ページ(範囲)323-329
ページ数7
ジャーナルWounds
26
11
出版ステータスPublished - 2014 11 1
外部発表はい

ASJC Scopus subject areas

  • 外科
  • 外科医療

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