Visualization of single membrane protein structure in stretched lipid bilayer suspended over nanowells

Youichi Shinozaki, Koji Sumitomo, Kazuaki Furukawa, Hidetoshi Miyashita, Yukihiro Tamba, Nahoko Kasai, Hiroshi Nakashima, Keiichi Torimitsu

研究成果: Article査読

12 被引用数 (Scopus)

抄録

In this study, we observed the topology of a single protein in a stretched lipid bilayer (membrane) suspended over a nanoscale well using a fast-scanning atomic force microscope (AFM). The membrane was located stably enough on the well to prevent the leakage of a liquid placed in the well, and it allowed us to observe membrane stretching using an AFM. We successfully observed the gradual stretching of the suspended membrane. We also observed single bacteriorhodopsin proteins in the stretched membrane, and found that they maintained their trimeric structure, but that the distances between the trimers increased.

本文言語English
論文番号027002
ジャーナルApplied Physics Express
3
2
DOI
出版ステータスPublished - 2010 2
外部発表はい

ASJC Scopus subject areas

  • 工学(全般)
  • 物理学および天文学(全般)

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