Visualization of microorganisms in bioprocesses

Tsuyoshi Yamaguchi, Kengo Kubota

研究成果: Chapter

2 被引用数 (Scopus)

抄録

Fluorescence in situ hybridization (FISH) is currently a standard technique for detecting, identifying, and enumerating microorganisms. The method allows for visualization of cell morphology as well as in situ localization of microorganisms at single-cell resolution. Although FISH has been used for more than 20 years, many challenges remain with regard to improving the technique for understanding microbial ecology and physiology. This chapter describes the recent developments in this field, such as improved sensitivity by catalyzed reporter deposition (CARD)-FISH and in situ DNA-hybridization chain reaction (HCR). Highly sensitive methods for detecting mRNA and/or functional genes are also described. Moreover, methods combining isotope probing to reveal microbial metabolic activities are introduced.

本文言語English
ホスト出版物のタイトルOptimization and Applicability of Bioprocesses
出版社Springer Singapore
ページ13-26
ページ数14
ISBN(電子版)9789811068638
ISBN(印刷版)9789811068621
DOI
出版ステータスPublished - 2018 1 2

ASJC Scopus subject areas

  • 医学(全般)
  • 健康専門職(全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 工学(全般)
  • 環境科学(全般)
  • 免疫学および微生物学(全般)

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