Visualization of enzymatic reaction in a microfluidic channel using chemical imaging sensor

Ko Ichiro Miyamoto, Yuji Hirayama, Torsten Wagner, Michael J. Schöning, Tatsuo Yoshinobu

研究成果: Article査読

17 被引用数 (Scopus)

抄録

The chemical imaging sensor is a semiconductor-based chemical sensor which is capable of visualizing the spatial distribution of chemical species. In this study, we applied the chemical imaging sensor to a dye-free method of visualizing an enzymatic reaction in a microfluidic channel. Microgel beads loaded with enzyme molecules were trapped in the microchannel. The substrate of the enzyme was injected from the upstream, and then the product of enzymatic reaction was detected in the downstream as pH change. The spatiotemporal change of pH distribution caused by the enzymatic reaction was successfully visualized. The possibility of chemical imaging inside a microchannel also suggests potential application, for example, to chromatography.

本文言語English
ページ(範囲)768-772
ページ数5
ジャーナルElectrochimica Acta
113
DOI
出版ステータスPublished - 2013 12 15

ASJC Scopus subject areas

  • 化学工学(全般)
  • 電気化学

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