Virus-mediated transduction of apolipoprotein E (ApoE)-Sendai develops lipoprotein glomerulopathy in ApoE-deficient mice

Yasushi Ishigaki, Shinichi Oikawa, Takashi Suzuki, Shinichi Usui, Kenta Magoori, Dong Ho Kim, Hiroyuki Suzuki, Jun Sasaki, Hironobu Sasano, Mitsuyo Okazaki, Takayoshi Toyota, Takao Saito, Tokuo T. Yamamoto

研究成果: Article査読

55 被引用数 (Scopus)

抄録

Lipoprotein glomerulopathy (LPG) is a unique renal disease characterized by thrombus-like substances in markedly dilated glomerular capillaries, dysbetalipoproteinemia, and elevated plasma concentrations of apoE. Recent studies identified several apoE mutations in patients with LPG, including apoE2(R145P) Sendal (apoE-Sendai). Virus-mediated transduction of apoE-Sendai in apoE-deficient hypereholesterolemic mice resulted in insufficient correction of hypercholesterolemia and a marked and temporal induction of plasma triglyceride levels. In vitro binding studies showed that apoE-Sendal has a reduced affinity for the low density lipoprotein receptor, suggesting that dysbetalipoproteinemia in LPG is caused by the apoE mutation. Furthermore, histological examination revealed marked intraglomerular depositions of apoE-containing lipoproteins in mice injected with apoE-Sendai virus. These LPG-like depositions were detected 6 days after virus injection and were sustained for at least 69 days. Our results demonstrated that apoE-Sendai is an etiological cause of LPG.

本文言語English
ページ(範囲)31269-31273
ページ数5
ジャーナルJournal of Biological Chemistry
275
40
DOI
出版ステータスPublished - 2000 10 6

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

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