vCJD prion acquires altered virulence through trans-species infection

Masahiro Asano, Shirou Mohri, James W. Ironside, Mamoru Ito, Norikazu Tamaoki, Tetsuyuki Kitamoto

研究成果: Article査読

45 被引用数 (Scopus)

抄録

Variant Creutzfeldt-Jakob disease (vCJD) appears to be caused by infection with the bovine spongiform encephalopathy (BSE) agent. To date, all patients with vCJD are homozygous for methionine at codon 129 of the PrP gene. To investigate the relationship between polymorphism at codon 129 and susceptibility to BSE or vCJD prions, we performed splenic follicular dendritic cell assay with humanized knock-in mice through peripheral infection. All humanized knock-in mice showed little or no susceptibility to BSE prions. Only the subset of humanized knock-in mice with codon 129 Met/Met genotype showed weak susceptibility by Western blotting. Surprisingly, we succeeded in the transmission of vCJD prions to humanized knock-in mice not only with codon 129 Met/Met but also with codon 129 Met/Val. Humanized knock-in mice with codon 129 Val/Val were not susceptible. The results suggest that human heterozygotes at codon 129 are also at risk for secondary infection with vCJD.

本文言語English
ページ(範囲)293-299
ページ数7
ジャーナルBiochemical and biophysical research communications
342
1
DOI
出版ステータスPublished - 2006 3月 31

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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