Variations and Distributions of O-Glycosidically Linked Sugar Chains in Bovine κ-Casein

Tadao Saito, Takatoshi Itoh

研究成果: Article査読

118 被引用数 (Scopus)

抄録

The variation and distribution of O-glycosidically linked sugar chains in κ-casein from bovine mature milk were analyzed by HPLC, fast atom bombardment mass spectrometry, and 13C nuclear magnetic resonance. A monosaccharide alditol (N-acetylgalactosaminitol) and four oligosaccharide alditols [a neutral disaccharide (galactosyl-β1-3N-acetylgalactosaminitol), two acidic trisaccharides (straight chain: N-acetylneuraminyl α2-3galactosyl-β1-3N-acetylgalactosaminitol and branch chain: galactosyl β1-3 (N-acetylneuraminyl α2-6)N-acetylgalactosaminitol), and an acidic tetrasaccharide (N-acetylneuraminyl α2-3galactosyl-β1-3(N-acetylgalactosaminyl α2-6)N-acetylgalactosaminitol] were identified as sugar chains linking on normal bovine κ-casein. The most dominant sugar chain was an acidic tetrasaccharide. The four oligosaccharide chains were identical to those already found, but N-acetylgalactosamine was a newly identified sugar chain linking on κ-casein. The distribution of monosaccharide, disaccharide, trisaccharide (straight), trisaccharide (branched), and tetrasaccharide chains were determined by HPLC to be .8, 6.3, 18.4, 18.5, and 56.0%, respectively (means of five κ-caseins).

本文言語English
ページ(範囲)1768-1774
ページ数7
ジャーナルJournal of Dairy Science
75
7
DOI
出版ステータスPublished - 1992

ASJC Scopus subject areas

  • 食品科学
  • 動物科学および動物学
  • 遺伝学

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