Use of native gels to measure protein binding to SSB.

Jin Inoue, Tsutomu Mikawa

研究成果: Article査読

抄録

We describe a procedure to detect protein binding to SSB by polyacrylamide gel electrophoresis under non-denaturing conditions. As an example, we show the interaction of Thermus thermophilus (Tth) SSB with its cognate RecO protein. The interaction is detected as decay of the band corresponding to SSB by addition of RecO. We also demonstrate analysis of the RecO-RecR interaction as another example of this method.

本文言語English
ページ(範囲)175-182
ページ数8
ジャーナルMethods in molecular biology (Clifton, N.J.)
922
出版ステータスPublished - 2012
外部発表はい

ASJC Scopus subject areas

  • Medicine(all)

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