Up-regulation of PSF1 promotes the growth of breast cancer cells

Izumi Nakahara, Mamiko Miyamoto, Tatsuhiro Shibata, Sadako Akashi-Tanaka, Takayuki Kinoshita, Kaoru Mogushi, Kohtaro Oda, Masaya Ueno, Nobuyuki Takakura, Hiroshi Mizushima, Hiroshi Tanaka, Tsutomu Ohta

研究成果: Article査読

29 被引用数 (Scopus)

抄録

PSF1 is a subunit of the GINS complex that functions along with the MCM2-7 complex and Cdc45 in eukaryotic DNA replication. Although mammalian PSF1 is predominantly expressed in highly proliferating cells and organs, little is known about the roles of PSF1 in mature cells or cancer cells. We found that PSF1 was expressed at relatively high levels in breast tumor cells, but at low levels in normal breast cells. Knockdown of PSF1 expression using small interfering RNA (siRNA) slowed the growth of breast cancer cell lines by delaying DNA replication but did not affect proliferation of normal human mammary epithelial cells. Reduced PSF1 expression also inhibited anchorage-independent growth in breast cancer cell lines. These results suggest that PSF1 over-expression is specifically involved in breast cancer cell growth. Therefore, PSF1 inhibition might provide new therapeutic approaches for breast cancer.

本文言語English
ページ(範囲)1015-1024
ページ数10
ジャーナルGenes to Cells
15
10
DOI
出版ステータスPublished - 2010 10

ASJC Scopus subject areas

  • 遺伝学
  • 細胞生物学

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