Up-regulation of protein serine/threonine phosphatase type 2C during 1α,25-dihydroxyvitamin D3-induced monocytic differentiation of leukemic HL-60 cells

Masakatsu Nishikawa, Serdar B. Omay, Keiji Nakai, Hisakazu Kihira, Takayasu Kobayashi, Shinri Tamura, Hiroshi Shiku

研究成果: Article査読

12 被引用数 (Scopus)

抄録

Treatment with 20 nM 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) caused a progressive increase in the activity of Mg2+-dependent protein serine/threonine phosphatase type 2C (PP2C) in subcellular fractions of HL-60 cells, whereas PP2C activity was relatively constant throughout all-trans retinoic acid-induced (1 μM) granulocytic differentiation. The increase in PP2C activity appeared to parallel the 1,25(OH)2D3-induced phenotypic and functional changes in HL-60 cells. Immunoblot and Northern blot analysis indicated that the increase in PP2C activity corresponded to the increased expression of PP2C protein, which was preceded by an increase in the level of mRNA for PP2Cβ. No mRNA for PP2Cα was detected in resting or 1,25(OH)2D3-stimulated HL-60 cells. These results suggest that the increased expression of PP2C is related with the 1,25(OH)2D3-induced monocytic differentiation of HL-60 cells.

本文言語English
ページ(範囲)299-303
ページ数5
ジャーナルFEBS Letters
375
3
DOI
出版ステータスPublished - 1995 11 20

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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