Unique properties of a renal sulfotransferase, St1d1, in dopamine metabolism

Miki Shimada, Reiko Terazawa, Yoshiteru Kamiyama, Wataru Honma, Kiyoshi Nagata, Yasushi Yamazoe

    研究成果: Article査読

    16 被引用数 (Scopus)

    抄録

    Although catecholamine sulfation is higher in the kidney than in the liver of mice, no detectable amounts of previously reported sulfotransferases (STs) such as St1a, St1b, St1c, and St1e were expressed in mouse kidney cytosols. A new sulfotransferase (St1d1) cDNA was isolated from kidney cDNA library of BALB/c strain by reverse transcription-polymerase chain reaction (RT-PCR) using information from expressed sequence tags (EST) database. The cDNA sequence resembled that of cDNA reported previously (AA238910) (Sakakibara et al., 1998) but differed in two amino acids, 206Q/K and 216Y/F, in the deduced amino acid sequence. The St1d1 expressed had unique substrate specificities for catecholamine derivatives, which preferred their deaminated metabolites rather than their parent amines. St1d1 showed the highest activity toward 3,4-dihydroxyphenylacetic acid (230.2 ± 2.69 nmol/mg/min) among the examined substrate. St1d1 protein was abundant in kidney, followed by liver, lung, and uterus. Furthermore, an addition of anti-St1d1 serum in the cytosolic reaction mixture resulted in complete inhibition of the sulfotransferase activity suggesting a major role of St1d1 on catecholamine sulfations. No human ST1D ortholog was detected at both mRNA and protein levels, although ST1A5 selectively catalyzing parent amine sulfation was detected in human kidney. These results indicate the functional basis of sulfation and the clear species difference on renal catecholamine metabolisms in mice and humans.

    本文言語English
    ページ(範囲)808-814
    ページ数7
    ジャーナルJournal of Pharmacology and Experimental Therapeutics
    310
    2
    DOI
    出版ステータスPublished - 2004 8 1

    ASJC Scopus subject areas

    • Molecular Medicine
    • Pharmacology

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