Unidirectional signal propagation in primary neurons micropatterned at a single-cell resolution

H. Yamamoto, R. Matsumura, H. Takaoki, S. Katsurabayashi, A. Hirano-Iwata, M. Niwano

研究成果: Article査読

14 被引用数 (Scopus)

抄録

The structure and connectivity of cultured neuronal networks can be controlled by using micropatterned surfaces. Here, we demonstrate that the direction of signal propagation can be precisely controlled at a single-cell resolution by growing primary neurons on micropatterns. To achieve this, we first examined the process by which axons develop and how synapses form in micropatterned primary neurons using immunocytochemistry. By aligning asymmetric micropatterns with a marginal gap, it was possible to pattern primary neurons with a directed polarization axis at the single-cell level. We then examined how synapses develop on micropatterned hippocampal neurons. Three types of micropatterns with different numbers of short paths for dendrite growth were compared. A normal development in synapse density was observed when micropatterns with three or more short paths were used. Finally, we performed double patch clamp recordings on micropatterned neurons to confirm that these synapses are indeed functional, and that the neuronal signal is transmitted unidirectionally in the intended orientation. This work provides a practical guideline for patterning single neurons to design functional neuronal networks in vitro with the direction of signal propagation being controlled.

本文言語English
論文番号043703
ジャーナルApplied Physics Letters
109
4
DOI
出版ステータスPublished - 2016 7 25

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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