Ultrasonically induced cell damage enhanced by photofrin II: Mechanism of sonodynamic activation

N. Yumita, S. Umemura, R. Nishigaki

研究成果: Article査読

51 被引用数 (Scopus)

抄録

Ultrasonically induced cell damage and active oxygen generation with photofrin II (PF) were compared in the same in vitro insonation setup. Materials and methods: Sarcoma 180 cells suspended in air-saturated PBS were exposed to ultrasound for up to 60 seconds in the presence and absence of PF. The viability was determined by the Trypan Blue exclusion test. Ultrasonically induced active oxygen generation in the presence and absence of PF in air-saturated aqueous solutions of 50 mM 2,2,6,6-tetramethyl-4-piperidone was detected by electron spin resonance (ESR) spectrum. Results: Significant enhancement of the rates of both ultrasonically induced cell damage and nitroxide generation was demonstrated with 20-80 μg/ml PF. Both rates correlated very well. The enhancement of both rates with PF was suppressed by 10 mM histidine. Conclusion: These results may suggest that ultrasonically generated active oxygen plays a primary role in ultrasonically induced cell damage in the presence of PF.

本文言語English
ページ(範囲)425-429
ページ数5
ジャーナルIn Vivo
14
3
出版ステータスPublished - 2000

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 薬理学

フィンガープリント

「Ultrasonically induced cell damage enhanced by photofrin II: Mechanism of sonodynamic activation」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル