Tyrosine phosphorylation of phospholipase Cγ in c-met/HGF receptor-stimulated hepatocytes: Comparison with HepG2 hepatocarcinoma cells

Yukio Okano, Kensaku Mizuno, Sinji Osada, Toshikazu Nakamura, Yoshinori Nozawa

研究成果: Article査読

49 被引用数 (Scopus)

抄録

Hepatocyte growth factor (HGF) stimulates inositol 1,4,5-trisphosphate (InsP3) formation in rat primary cultured hepatocytes, which is inhibited by the pretreatment with a tyrosine kinase inhibitor, genistein. This lnsP3 production was coincident with tyrosine phosphorylation of phospholipase Cγ (PLCγ), detected in immunoprecipitates with anti-PLCγ, suggesting activation mechanism of PLCγ by tyrosine phosphorylation. However, in human hepatocarcinoma HepG2 cells, HGF, which suppresses cell growth, causes neither phosphorylation of PLCγ nor lnsP3 formation. The results suggests that PLCγ in normal hepatocytes was activated by HGF through tyrosine kinase of HGF receptor.

本文言語English
ページ(範囲)842-848
ページ数7
ジャーナルBiochemical and biophysical research communications
190
3
DOI
出版ステータスPublished - 1993 2月 15
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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