Type X collagen is not localized in hypertrophic or calcified cartilage in the developing rat trachea

Yasuyuki Sasano, Ichiro Takahashi, Itaru Mizoguchi, Manabu Kagayama, Hiroko Takita, Yoshinori Kuboki

研究成果: Article査読

8 被引用数 (Scopus)

抄録

Our previous studies have shown that rat tracheal chondrocytes become larger and hypertrophic, and that the cartilage matrix calcifies during development. Type X collagen is a short collagen molecule identified in hypertrophic and calcified cartilage in the growth plate of long bones during endochondral ossification. The present study was designed to investigate the distribution of type X collagen in rat tracheal cartilage during development before and after hypertrophization and calcification. Tracheas from postnatal Wistar rats, newborn, and at 4, 8 and 10 weeks were fixed along with hind limbs from newborn rats. Serial sections were made and adjacent sections were processed for von Kossa staining or immunohistochemistry for type X collagen. In addition, the immunoreactivity to type II collagen was examined as a control. The anti-type X collagen antibody stained hypertrophic and/or calcified cartilage in the newborn rat tibia. The immunoreaction for type X collagen was localized in the uncalcified peripheral region of tracheal cartilage in 4, 8 and 10-week-old rats. In contrast, the anti-type X collagen antibody did not show immunoreactivity to hypertrophic or calcified cartilage in the central region of the 10-week-old rat tracheal cartilage. The present study has suggested that type X collagen is not involved in hypertrophization of chondrocytes or calcification of the matrix in developing rat tracheal cartilage.

本文言語English
ページ(範囲)399-403
ページ数5
ジャーナルAnatomy and Embryology
197
5
DOI
出版ステータスPublished - 1998 5 26

ASJC Scopus subject areas

  • 解剖学
  • 胎生学
  • 発生生物学
  • 細胞生物学

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