Two methods of temperature control for single-molecule measurements

Matthew A.B. Baker, Yuichi Inoue, Kuniaki Takeda, Akihiko Ishijima, Richard M. Berry

研究成果: Article査読

10 被引用数 (Scopus)

抄録

Modern single-molecule biophysical experiments require high numerical aperture oil-immersion objectives in close contact with the sample. We introduce two methods of high numerical aperture temperature control which can be implemented on any microscope: objective temperature control using a ring-shaped Peltier device, and stage temperature control using a fluid flow cooling chip in close thermal contact with the sample. We demonstrate the efficacy of each system by showing the change in speed with temperature of two molecular motors, the bacterial flagellar motor and skeletal muscle myosin.

本文言語English
ページ(範囲)651-660
ページ数10
ジャーナルEuropean Biophysics Journal
40
5
DOI
出版ステータスPublished - 2011 5

ASJC Scopus subject areas

  • Biophysics

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