Two isoforms of the rat kidney EP3 receptor derived by alternative RNA splicing: Intrarenal expression co-localization

Kazuhisa Takeuchi, Nobuyuki Takahashi, Takaaki Abe, Keishi Abe

研究成果: Article査読

42 被引用数 (Scopus)

抄録

Two different clones, named rEP3A (∼2.2 kb) and rEP3B (∼5.2 kb), were isolated from a rat kidney cDNA library by a homology screening approach. rEP3A was shown to encode the rat kidney prostaglandin E receptor EP3 subtype (rEP3A receptor) (Takeuchi, K. et al. B.B.R.C. (1993) 194: 885). rEP3B receptor differs only in its carboxyl-terminal tail (Ile-336 to Pro-364) from rEP3A receptor. Southern blot analysis of genomic DNA has suggested that the EP3 receptor gene is a single copy gene. RT-PCR using microdissected nephron segments showed co-expression of both receptor mRNAs specifically in distal nephron segments such as mTAL, cTAL, CCD and IMCD, whereas no significant expression of both receptor mRNAs was detected from GL, PCT, and PST. In conclusion, we have cloned an isoform of the rat kidney EP3 receptor, rEP3B receptor. rEP3A and rEP3B receptors are suggested to be derived by alternative RNA splicing, and both receptors are co-localized to distal tubules exerting an effect on water and electrolyte metabolism.

本文言語English
ページ(範囲)834-840
ページ数7
ジャーナルBiochemical and biophysical research communications
199
2
DOI
出版ステータスPublished - 1994 3 15

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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