Trigonelline was isolated from Aeschynomene indica as a bioactive substance for nyctinasty. The compound was quite effective for leaf-closing of this species at 1 × 10-7 M in the daytime, but not for the nyctinastic species Cassia mimosoides and Mimosa pudica. It competed with indole-3-acetic acid (IAA) which is effective for leaf-opening. These results suggest that trigonelline may be concerned with the circadian rhythm of A. indica.
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