Transscleral controlled delivery of geranylgeranylaceton using a polymeric device protects rat retina against light injury

研究成果: Chapter

5 被引用数 (Scopus)

抄録

We evaluated the effects of a transscleral drug delivery device, consisting of a reservoir and controlled-release cover, which were made of photopolymerized polyethylene glycol dimethacrylate and triethylene glycol dimethacrylate, combined at different ratios. Geranylgeranylacetone (GGA), a heat-shock protein (HSP) inducer, was loaded into the device. The GGA was released from the device under zero-order kinetics. At both 1 week and 4 weeks after device implantation on rat sclera, HSP70 gene and protein expression were up-regulated in the sclerachoroid- retinal pigment epithelium fraction of rat eyes treated with the GGA-loaded device compared with rat eyes treated with saline-loaded devices or eyes of nontreated rats. Flash electroretinograms were recorded 4 days after white light exposure (8000 lx for 18 h). Electroretinographic amplitudes of the a- and b-waves were preserved significantly in rats treated with GGA-loaded devices compared with rats treated with saline-loaded devices. Histological examination showed that the outer nuclear layer thickness was preserved in rats that had the GGA-loaded device. These results may show that transscleral GGA delivery using our device may offer an alternative method to treat retinal diseases.

本文言語English
ホスト出版物のタイトルAdvances in Experimental Medicine and Biology
出版社Springer New York LLC
ページ471-477
ページ数7
DOI
出版ステータスPublished - 2016 10 1

出版物シリーズ

名前Advances in Experimental Medicine and Biology
854
ISSN(印刷版)0065-2598
ISSN(電子版)2214-8019

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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