Translational downregulation of RBCL is operative in the coordinated expression of Rubisco genes in senescent leaves in rice

Yuji Suzuki, Amane Makino

研究成果: Article査読

28 被引用数 (Scopus)

抄録

Rubisco gene expression was examined in detail in rice (Oryza sativa L.) leaves at different positions, i.e. expanding, mature, and senescent leaves. Rubisco small subunit (RBCS) synthesis and RBCS mRNA levels were maximal in expanding leaves and gradually became lower in mature and senescent leaves, with declines in those of the large subunit (RBCL) being relatively slower. The amount of synthesized RBCL per unit level of RBCL mRNA and polysome loading of RBCL mRNA declined in senescent leaves, whereas such phenomena were not observed for RBCS. These results suggested that gene expression of RBCL is downregulated at the level of its translation when a balance between RBCL and RBCS expression is disturbed by leaf senescence. It has been suggested that RBCS protein is a positive regulator for RBCL mRNA level in expanding rice leaves, as judged from their stoichiometric relationship in RBCS transgenic rice plants. However, the ratio of the RBCL mRNA level to the amount of synthesized RBCS in senescent leaves was significantly higher than that in expanding leaves. Therefore, it is suggested that the decline in RBCL mRNA level in senescent leaves is not fully accounted for by that in the amount of synthesized RBCS. Effects of other factors such as the stability of RBCL mRNA may come into play.

本文言語English
ページ(範囲)1145-1152
ページ数8
ジャーナルJournal of experimental botany
64
4
DOI
出版ステータスPublished - 2013 3月
外部発表はい

ASJC Scopus subject areas

  • 生理学
  • 植物科学

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