Transient elevation of glucose increases arrhythmia susceptibility in non-diabetic rat trabeculae with non-uniform contraction

Masahito Miura, Tetsuya Handoh, Yuhto Taguchi, Taiki Hasegawa, Yui Takahashi, Natsuki Morita, Ayana Matsumoto, Chiyohiko Shindoh, Haruka Sato

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Background: In non-diabetic patients with acute coronary syndrome, stress hyperglycemia occasionally occurs and is related to their mortality. Whether transient elevation of glucose affects arrhythmia susceptibility in non-diabetic hearts with non-uniform contraction was examined. Methods and Results: Force, intracellular Ca2+ ([Ca2+]i), and membrane potential were measured in trabeculae from rat hearts. Non-uniform contraction was produced by a jet of paralyzing solution. Ca2+ waves and arrhythmias were induced by electrical stimulation (2.0 mmol/L [Ca2+]o). The activity of Ca2+/calmodulin-dependent protein kinaseII (CaMKII) was measured. An elevation of glucose from 150 to 400 mg/dL increased the velocity of Ca2+ waves and the number of spontaneous action potentials triggered by electrical stimulation. Besides, the elevation of glucose increased the CaMKII activity. In the presence of 1 μmol/L KN-93, the elevation of glucose did not increase the velocity of Ca2+ waves and the number of triggered action potentials. In addition, in the presence of 1 μmol/L autocamtide-2 related inhibitory peptide or 50 μmol/L diazo-5-oxonorleucine, the elevation of glucose did not increase the number of triggered action potentials. Furthermore, the elevation of glucose by adding L-glucose did not increase their number. Conclusions: In non-diabetic hearts with non-uniform contraction, transient elevation of glucose increases the velocity of Ca2+ waves by activating CaMKII,probably through glycosylation with O-linked β-N-acetylglucosamine, thereby increasing arrhythmia susceptibility.

本文言語English
ページ(範囲)551-558
ページ数8
ジャーナルCirculation Journal
84
4
DOI
出版ステータスPublished - 2020
外部発表はい

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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