Transgenesis by microparticle bombardment for live imaging of fluorescent proteins in Pristionchus pacificus germline and early embryos

Satoshi Namai, Asako Sugimoto

    研究成果: Article査読

    9 被引用数 (Scopus)

    抄録

    Pristionchus pacificus is a free-living nematode used as a model organism for evolutionary developmental and ecological biology. Although a transgenic technique to form complex arrays by microinjection has been established in P. pacificus, transgene expression from the array in the germline and early embryos tends to be silenced. Here, we established a method to integrate transgenes into the genome of P. pacificus using microparticle bombardment with hygromycin B selection. Additionally, we isolated a mutant exhibiting significantly lower autofluorescence in the germline and early embryos, facilitating visualization of transgene-derived fluorescent proteins for live imaging. Transgenic lines constructed using these tools successfully expressed GFP-tagged proteins in the germline and early embryos and enabled live imaging of chromosomes, microtubules, and centrosomes.

    本文言語English
    ページ(範囲)75-82
    ページ数8
    ジャーナルDevelopment Genes and Evolution
    228
    1
    DOI
    出版ステータスPublished - 2018 1月 1

    ASJC Scopus subject areas

    • 遺伝学
    • 発生生物学

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