Transformation of the industrial strain of Aspergillus oryzae with the homologous amdS gene as a dominant selectable marker

Katsuya Gomi, Katsuhiko Kitamoto, Chieko Kumagai

研究成果: Article

30 引用 (Scopus)

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A homologous transformation system has been developed for Aspergillus oryzae industrial strains using the A. oryzae acetamidase-encoding gene (amdS) as a dominant selectable marker. Although the transformation efficiency is relatively low (0.4-1 transformants per μg DNA), the transformants contained significantly high copy numbers of the plasmid integrated into the genome. In addition, the promoter of the amdS gene was replaced by the strong expression promoter of the α-amylase-encoding gene (amyB) from A. oryzae, thereby enhancing the selection of the transformants.

元の言語English
ページ(範囲)389-391
ページ数3
ジャーナルJournal of Fermentation and Bioengineering
74
発行部数6
DOI
出版物ステータスPublished - 1992 1 1
外部発表Yes

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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