Transferring genes into cultured mammalian embryos by electroporation

Masanori Takahashi, Tadashi Nomura, Noriko Osumi

研究成果: Review article査読

33 被引用数 (Scopus)

抄録

Mammalian whole embryo culture (WEC) was developed long before transgenic and gene targeted animals are widely used. Electroporation (EP) into cultured rodent embryos has expanded the potential to analyze gene functions in mammalian embryos by transferring exogenous plasmid vectors or small nucleotides in region- and stage-specific ways. This method is quite simple, and therefore enables us to analyze gene functions more quickly than genetic manipulation. In this review, we introduce combinatorial methods of WEC and EP, and summarize various applications in developmental neurobiology.

本文言語English
ページ(範囲)485-497
ページ数13
ジャーナルDevelopment Growth and Differentiation
50
6
DOI
出版ステータスPublished - 2008 8月

ASJC Scopus subject areas

  • 発生生物学
  • 細胞生物学

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