Transcytosis of murine-adapted bovine spongiform encephalopathy agents in an in vitro bovine M cell model

Kohtaro Miyazawa, Takashi Kanaya, Ikuro Takakura, Sachi Tanaka, Tetsuya Hondo, Hitoshi Watanabe, Michael T. Rose, Haruki Kitazawa, Takahiro Yamaguchi, Shigeru Katamine, Noriyuki Nishida, Hisashi Aso

研究成果: Article査読

20 被引用数 (Scopus)

抄録

Transmissible spongiform encephalopathies (TSE), including bovine spongiform encephalopathy (BSE), are fatal neurodegenerative disorders in humans and animals. BSE appears to have spread to cattle through the consumption of feed contaminated with BSE/scrapie agents. In the case of an oral infection, the agents have to cross the gut-epithelial barrier. We recently established a bovine intestinal epithelial cell line (BIE cells) that can differentiate into the M cell type in vitro after lymphocytic stimulation (K. Miyazawa, T. Hondo, T. Kanaya, S. Tanaka, I. Takakura, W. Itani, M. T. Rose, H. Kitazawa, T. Yamaguchi, and H. Aso, Histochem. Cell Biol. 133:125-134, 2010). In this study, we evaluated the role of M cells in the intestinal invasion of the murine-adapted BSE (mBSE) agent using our in vitro bovine intestinal epithelial model. We demonstrate here that M cell-differentiated BIE cells are able to transport the mBSE agent without inactivation at least 30-fold more efficiently than undifferentiated BIE cells in our in vitro model. As M cells in the follicle-associated epithelium are known to have a high ability to transport a variety of macromolecules, viruses, and bacteria from gut lumen to mucosal immune cells, our results indicate the possibility that bovine M cells are able to deliver agents of TSE, not just the mBSE agent.

本文言語English
ページ(範囲)12285-12291
ページ数7
ジャーナルJournal of virology
84
23
DOI
出版ステータスPublished - 2010 12

ASJC Scopus subject areas

  • 微生物学
  • 免疫学
  • 昆虫科学
  • ウイルス学

フィンガープリント

「Transcytosis of murine-adapted bovine spongiform encephalopathy agents in an in vitro bovine M cell model」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル