Tracing ancestral specificity of lectins: Ancestral sequence reconstruction method as a new approach in protein engineering

Tomohisa Ogawa, Tsuyoshi Shirai

    研究成果: Chapter

    3 被引用数 (Scopus)

    抄録

    Protein evolution is a process of molecular design leading to the diversity of functional proteins found in nature. Recent advances in bioinformatics and structural biology, in addition to recombinant protein expression techniques, enable us to analyze more directly the molecular evolution of proteins by a new method using ancestral sequence reconstruction (ASR), the so-called experimental molecular archaeology. ASR has been used to reveal molecular properties and structures correlating with changing geology, ecology, and physiology, and to identify the structure elements important to changing physiological functions to fill substantial gaps in the processes of protein evolution. In this chapter, we describe ASR as a new method of protein engineering studies, and their application to analyzing lectins, of which evolutionary processes and structural features contributing to molecular stability, specificity, and unique functions have been elucidated. Experimental molecular archeology using ASR and crystal structures of full-length ancestral proteins is useful in understanding the evolutionary process of the functional and structural diversified lectins by tracing ancestral specificities.

    本文言語English
    ホスト出版物のタイトルLectins
    ホスト出版物のサブタイトルMethods and Protocols
    出版社Humana Press Inc.
    ページ539-551
    ページ数13
    ISBN(印刷版)9781493912919
    DOI
    出版ステータスPublished - 2014

    出版物シリーズ

    名前Methods in Molecular Biology
    1200
    ISSN(印刷版)1064-3745

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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