Toxoplasma gondii: Sensitive and rapid detection of infection by loop-mediated isothermal amplification (LAMP) method

Houshuang Zhang, Oriel M.M. Thekisoe, Gabriel O. Aboge, Hisako Kyan, Junya Yamagishi, Noboru Inoue, Yoshifumi Nishikawa, Satoshi Zakimi, Xuenan Xuan

研究成果: Article査読

75 被引用数 (Scopus)

抄録

Loop-mediated isothermal amplification (LAMP) method amplifies DNA with high specificity, sensitivity and rapidity. In this study, we used a conserved sequence in the 200- to 300-fold repetitive 529 bp gene of Toxoplasma gondii to design primers for LAMP test. Detection limit of T. gondii LAMP assay with the primers is 1 pg/μL of T. gondii DNA, which was evaluated using 10-fold serially diluted DNA of cultured parasites. Furthermore, LAMP and conventional PCR methods were applied for amplification of the T. gondii DNA extracted from the lymph nodes taken from pigs which were suspected to be Toxoplasma infection. As a result, 76.9% (70/91) and 85.7% (78/91) of the samples were positive on PCR and LAMP analyzes, respectively. Therefore, the LAMP has a potential to be applied as an alternative molecular diagnostic tool for detection of T. gondii infection from veterinary samples. This is the first study, which applies the LAMP method to diagnose Toxoplasma from veterinary samples.

本文言語English
ページ(範囲)47-50
ページ数4
ジャーナルExperimental Parasitology
122
1
DOI
出版ステータスPublished - 2009 5月
外部発表はい

ASJC Scopus subject areas

  • 寄生虫科
  • 免疫学
  • 感染症

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