Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis-Menten-like kinetics for cell culturing

S. Takenaga; C. Herrera; C. F. Werner; M. Biselli; V. Thönnessen; Th Schnitzler; P. Öhlschläger; F. N. Almajhdi; T. Wagner; M. J. Schöning

doi:10.1002/pssa.201330464

Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis-Menten-like kinetics for cell culturing

S. Takenaga, C. Herrera, C. F. Werner, M. Biselli, V. Thönnessen, Th Schnitzler, P. Öhlschläger, F. N. Almajhdi, T. Wagner, M. J. Schöning

研究成果: Article › 査読

5 被引用数 (Scopus)

抄録

The metabolic activity of Chinese hamster ovary (CHO) cells was observed using a light-addressable potentiometric sensor (LAPS). The dependency toward different glucose concentrations (17-200 mM) follows a Michaelis-Menten kinetics trajectory with K_m = 32.8 mM, and the obtained K_m value in this experiment was compared with that found in literature. In addition, the pH shift induced by glucose metabolism of tumor cells transfected with the HPV-16 genome (C3 cells) was successfully observed. These results indicate the possibility to determine the tumor cells metabolism with a LAPS-based measurement device.

本文言語	English
ページ（範囲）	1410-1415
ページ数	6
ジャーナル	Physica Status Solidi (A) Applications and Materials Science
巻	211
号	6
DOI	https://doi.org/10.1002/pssa.201330464
出版ステータス	Published - 2014 6月
外部発表	はい

ASJC Scopus subject areas

電子材料、光学材料、および磁性材料
凝縮系物理学
表面および界面
表面、皮膜および薄膜
電子工学および電気工学
材料化学

文献へのアクセス

10.1002/pssa.201330464

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引用スタイル

Takenaga, S., Herrera, C., Werner, C. F., Biselli, M., Thönnessen, V., Schnitzler, T., Öhlschläger, P., Almajhdi, F. N., Wagner, T., & Schöning, M. J. (2014). Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis-Menten-like kinetics for cell culturing. Physica Status Solidi (A) Applications and Materials Science, 211(6), 1410-1415. https://doi.org/10.1002/pssa.201330464

Takenaga, S, Herrera, C, Werner, CF, Biselli, M, Thönnessen, V, Schnitzler, T, Öhlschläger, P, Almajhdi, FN, Wagner, T & Schöning, MJ 2014, 'Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis-Menten-like kinetics for cell culturing', Physica Status Solidi (A) Applications and Materials Science, vol. 211, no. 6, pp. 1410-1415. https://doi.org/10.1002/pssa.201330464

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abstract = "The metabolic activity of Chinese hamster ovary (CHO) cells was observed using a light-addressable potentiometric sensor (LAPS). The dependency toward different glucose concentrations (17-200 mM) follows a Michaelis-Menten kinetics trajectory with Km = 32.8 mM, and the obtained Km value in this experiment was compared with that found in literature. In addition, the pH shift induced by glucose metabolism of tumor cells transfected with the HPV-16 genome (C3 cells) was successfully observed. These results indicate the possibility to determine the tumor cells metabolism with a LAPS-based measurement device.",

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AU - Werner, C. F.

AU - Biselli, M.

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