Tol2-mediated transgenesis, gene trapping, enhancer trapping, and the GAL4-UAS system

Gembu Abe, Maximilliano L. Suster, Koichi Kawakami

研究成果: Chapter

26 被引用数 (Scopus)

抄録

The Tol2 transposable element was originally found in the genome of the Japanese medaka fish (Oryzias latipes). Tol2 contains a gene encoding an active transposase that can catalyze DNA transposition in vertebrate cells. In zebrafish, Tol2 generates genomic integrations in the germ cells very efficiently. By using the Tol2 transposition system, we have developed important genetic methods including transgenesis, gene trapping, enhancer trapping, and the Gal4-UAS system in zebrafish. In this chapter, we describe how these methods can be performed.

本文言語English
ホスト出版物のタイトルMethods in Cell Biology
出版社Academic Press Inc.
ページ23-49
ページ数27
DOI
出版ステータスPublished - 2011
外部発表はい

出版物シリーズ

名前Methods in Cell Biology
104
ISSN(印刷版)0091-679X

ASJC Scopus subject areas

  • Cell Biology

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