Tissue distribution and characterization of peptide C-terminal α-amidating activity in rat

Junichiro Sakata, Kensaku Mizuno, Hisayuki Matsuo

研究成果: Article査読

53 被引用数 (Scopus)

抄録

The C-terminal α-amide formation of peptides is one of the most important events in prohormone processing. Recently, we developed a simple and sensitive assay for detecting α-amidating activity in tissues by using (125I)-Ac-Tyr-Phe-Gly as a substrate. Using this assay method, we have determined the tissue distribution of α-amidating enzyme activity in adult male rat. High concentrations of α-amidating activity were found in pituitary, brain, thyoid, gastrointestinal tract, pancreas, heart, submaxillary glands and parotid glands. Alpha-amidating enzyme activities in all tissues examined exhibit very similar copper and ascorbate requirements, pH dependence, and behavior on gel-filtration.

本文言語English
ページ(範囲)230-236
ページ数7
ジャーナルBiochemical and biophysical research communications
140
1
DOI
出版ステータスPublished - 1986 10 15
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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