The C-terminal α-amide formation of peptides is one of the most important events in prohormone processing. Recently, we developed a simple and sensitive assay for detecting α-amidating activity in tissues by using (125I)-Ac-Tyr-Phe-Gly as a substrate. Using this assay method, we have determined the tissue distribution of α-amidating enzyme activity in adult male rat. High concentrations of α-amidating activity were found in pituitary, brain, thyoid, gastrointestinal tract, pancreas, heart, submaxillary glands and parotid glands. Alpha-amidating enzyme activities in all tissues examined exhibit very similar copper and ascorbate requirements, pH dependence, and behavior on gel-filtration.
|ジャーナル||Biochemical and biophysical research communications|
|出版ステータス||Published - 1986 10 15|
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