TY - JOUR
T1 - THz irradiation inhibits cell division by affecting actin dynamics
AU - Yamazaki, Shota
AU - Ueno, Yuya
AU - Hosoki, Ryosuke
AU - Saito, Takanori
AU - Idehara, Toshitaka
AU - Yamaguchi, Yuusuke
AU - Otani, Chiko
AU - Ogawa, Yuichi
AU - Harata, Masahiko
AU - Hoshina, Hiromichi
N1 - Funding Information:
This work was supported by Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Numbers JP19K15812, JP20H05378, JP21H02151, JP20K21261, JP21H04746, the JSPS Core-to- Core Program (Advanced Research Networks) entitled "Establishment of international agricultural immunology research-core for a quantum improvement in food safety, the RIKEN-AIST Joint Research Fund (Semi-full research), the Cooperative Research Program of Research Center for Development of Far-Infrared Region University of Fukui (R02FIRDM022A, R03FIRDG018A) and the Japan-Czech Republic Research Cooperative Program between JSPS and CAS grant number JPJSBP120202501. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2021 Yamazaki et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2021/8
Y1 - 2021/8
N2 - Biological phenomena induced by terahertz (THz) irradiation are described in recent reports, but underlying mechanisms, structural and dynamical change of specific molecules are still unclear. In this paper, we performed time-lapse morphological analysis of human cells and found that THz irradiation halts cell division at cytokinesis. At the end of cytokinesis, the contractile ring, which consists of filamentous actin (F-actin), needs to disappear; however, it remained for 1 hour under THz irradiation. Induction of the functional structures of F-actin was also observed in interphase cells. Similar phenomena were also observed under chemical treatment (jasplakinolide), indicating that THz irradiation assists actin polymerization. We previously reported that THz irradiation enhances the polymerization of purified actin in vitro; our current work shows that it increases cytoplasmic F-actin in vivo. Thus, we identified one of the key biomechanisms affected by THz waves.
AB - Biological phenomena induced by terahertz (THz) irradiation are described in recent reports, but underlying mechanisms, structural and dynamical change of specific molecules are still unclear. In this paper, we performed time-lapse morphological analysis of human cells and found that THz irradiation halts cell division at cytokinesis. At the end of cytokinesis, the contractile ring, which consists of filamentous actin (F-actin), needs to disappear; however, it remained for 1 hour under THz irradiation. Induction of the functional structures of F-actin was also observed in interphase cells. Similar phenomena were also observed under chemical treatment (jasplakinolide), indicating that THz irradiation assists actin polymerization. We previously reported that THz irradiation enhances the polymerization of purified actin in vitro; our current work shows that it increases cytoplasmic F-actin in vivo. Thus, we identified one of the key biomechanisms affected by THz waves.
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U2 - 10.1371/journal.pone.0248381
DO - 10.1371/journal.pone.0248381
M3 - Article
C2 - 34339441
AN - SCOPUS:85111744686
VL - 16
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 8 August
M1 - e0248381
ER -