Thymocyte proliferation and differentiation in human granulocyte-macrophage colony-stimulating factor receptor transgenic mice

Yuko Yasuda, Ichiko Nishijima, Sumiko Watanabe, Ken Ichi Arai

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Thymocytes display varying responses to cytokines depending on their stage of differentiation. Whether these responses are due to stage-specific cytokine receptor expression or to down-stream signaling mechanisms is unknown. We examined the relationship between receptor expression and thymocyte proliferation or differentiation by using thymocytes from transgenic mice that constitutively expressed the human granulocyte-macrophage colony stimulating factor (hGM-CSF) receptor. Transgenic CD4-CD8-, CD4+CD8-, and CD4-CD8+ thymocyte populations expressing the hGM-CSF receptor proliferated when cultured with hGM-CSF, whereas CD4+CD8+ cells failed to proliferate despite expressing this receptor. We next examined the effect of hGM-CSF receptor signaling on thymocyte differentiation in fetal thymic organ culture supporting a full program of T cell development in vitro. Addition of hGM-CSF to the transgenic fetal thymic organ culture resulted in failure of CD4-CD8- cells to differentiate into CD4+CD8+ cells. To investigate this maturational inhibition more closely, we repopulated wild-type fetal lobes with sorted pro-T, pre-T or post pre-T precursor cells from hGM-CSF receptor transgenic mice. In these cultures hGM-CSF blocked both pro-T and pre-T cell differentiation, whereas the more mature post pre-T cells differentiated normally. These results suggest that hGM-CSF receptor signaling during thymocyte differentiation causes stage-specific inhibition of precursor cell maturation. In addition, repopulation studies of transgenic fetal lobes with sorted wild-type thymocyte precursors indicated that hGM-CSF inhibited proliferation and differentiation of the wild-type precursors, suggesting a secondary effect via transgenic stromal cells.

本文言語English
ページ(範囲)S87-S96
ジャーナルJournal of Allergy and Clinical Immunology
100
6 SUPPL.
DOI
出版ステータスPublished - 1997
外部発表はい

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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