Thymidine phosphorylase expressed in macrophages enhances antitumor effect of 5′-deoxy-5-fluorouridine on human colorectal carcinoma cells

Jimin Zhang, Takayuki Mizoi, Nobuhiko Harada, Ken Ichi Shiiba, Kikuo Miyagawa, Seiki Matsuno, Iwao Sasaki

研究成果: Article査読

9 被引用数 (Scopus)

抄録

Background: Thymidine phosphorylase (dThdPase) is a key enzyme in the activation of the pro-drugs of 5-fluorouracil (5-FU), 5′-deoxy-5-fluoroufidine (5′-DFUR) and capecitabine. In colorectal carcinoma (CRC), the major cells expressing dThdPase have been shown to be stromal cells, particularly macrophages. Materials and Methods: The present study was designed to clarify whether dThdPase expressed in macrophage-like cell lines, THP-1 and U937, and monocyte-rich mononuclear cells (MoMNCs) from human peripheral blood can modulate the antitumor effect of 5′-DFUR on CRC cells. Results: dThdPase protein was found in THP-1 and U937 by ELISA, while little or no dThdPase could be detected in the CRC cell lines tested. Incubation of 5′-DFUR with the macrophage-like cells significantly enhanced the antitumor effect of 5′-DFUR in a 5-DFUR sensitivity assay compared with untreated 5′-DFUR. MoMNCs also showed a similar effect. When the media containing 5′-DFUR was treated with either THP-1 or U937 cells, detectable levels of 5-FU could be measured in the treated media. Conclusion: These data suggest that macrophages convert 5′-DFUR to 5-FU and release the converted 5-FU, resulting in an enhancement of the antitumor effect of 5′-DFUR.

本文言語English
ページ(範囲)323-329
ページ数7
ジャーナルAnticancer research
23
1 A
出版ステータスPublished - 2003 1
外部発表はい

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

フィンガープリント

「Thymidine phosphorylase expressed in macrophages enhances antitumor effect of 5′-deoxy-5-fluorouridine on human colorectal carcinoma cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル