Thermosensitive gel formation of novel polypeptides containing a collagen-derived Pro-Hyp-Gly sequence and an elastin-derived Val-Pro-Gly-Val-Gly sequence

Yasushi Morihara, Shin Ichi Ogata, Masanobu Kamitakahara, Chikara Ohtsuki, Masao Tanihara

研究成果: Article査読

20 被引用数 (Scopus)

抄録

A triple-helix-forming collagen model peptide, (prolyl-trans-4-hydroxyprolyl-glycyl)10 [(Pro-Hyp-Gly)10], and a thermosensitive elastin-derived pentapeptide, valyl-prolyl-glycyl-valyl-glycyl (Val-Pro-Gly-Val-Gly), were copolymerized in various mole ratios using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride and 1-hydroxy-benzotriazole in dimethyl sulfoxide at 20 °C. All of the obtained polypeptides have molecular weight higher than 10 3 and contain a triple-helical structure, and showed an inverse phase transition from transparent solution to turbid suspension in response to a rise in temperature. The lower critical solution temperature of the polypeptide solution decreased upon increasing the content of Val-Pro-Gly-Val-Gly. Furthermore, polypeptides containing 82-86 mol % of Val-Pro-Gly-Val-Gly in composition showed reversible gel formation, suggesting that (Pro-Hyp-Gly)10 acts as a hydrated unit and Val-Pro-Gly-Val-Gly acts as a thermosensitive crosslinking point. These biodegradable thermosensitive polypeptides may be useful for biomedical applications, including, as a scaffold for tissue regeneration.

本文言語English
ページ(範囲)6048-6056
ページ数9
ジャーナルJournal of Polymer Science, Part A: Polymer Chemistry
43
23
DOI
出版ステータスPublished - 2005 12月 1
外部発表はい

ASJC Scopus subject areas

  • ポリマーおよびプラスチック
  • 有機化学
  • 材料化学

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