Theonezolide A, a novel marine macrolide, induces drastic shape change in rabbit platelets by reorganization of microtubules

Minori Mitsui-Saito, Satoko Ohkubo, Yutaro Obara, Teruyuki Yanagisawa, Jun'Ichi Kobayashi, Yasushi Ohizumi, Norimichi Nakahata

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Theonezolide A, a marine macrolide, and thrombin caused a shape change followed by an aggregation in the rabbit platelets. Theonezolide A-induced platelet shape change, estimated by a decrease in light transmission, appeared to a greater extent than thrombin-induced one. Morphological studies using an electron microscope showed that theonezolide A changed platelet shape with various numbers of long pseudopods, loosing their discoid shape. Theonezolide A-induced shape change was inhibited by a microtubule-stabilizing agent, taxol, but not by an actin-depolymerizing agent, cytochalasin B. In contrast, thrombin-induced shape change was inhibited by cytochalasin B but not by taxol. Confocal fluorescence microscopy showed that circumferential microtubule bundle disappeared in the platelets treated with theonezolide A. Theonezolide A had no direct effect on polymerization of microtubules isolated from bovine brain, indicating that it indirectly causes microtubule reorganization. These results suggest that theonezolide A induces drastic shape change through reorganization of microtubules in rabbit platelets. Thus, theonezolide A is a useful drug to examine microtubule reorganization in the cells.

本文言語English
ページ(範囲)133-138
ページ数6
ジャーナルThrombosis Research
108
2-3
DOI
出版ステータスPublished - 2002 11 1

ASJC Scopus subject areas

  • 血液学

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