The visualization of the mineral and protein distribution in the same histological sections of rat calcified growth plate cartilage

Miyuki Mayanagi; Megumi Nakamura; Akiko Henmi; Yasuyuki Sasano

doi:10.1016/j.job.2022.08.005

The visualization of the mineral and protein distribution in the same histological sections of rat calcified growth plate cartilage

Miyuki Mayanagi, Megumi Nakamura, Akiko Henmi, Yasuyuki Sasano

歯学研究科・歯科学専攻

研究成果: Article › 査読

抄録

Objective: To determine whether the combination of scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDX) and immunostaining would visualize the mineral and protein distribution in the same histological sections. Methods: Paraffin sections of fixed rat hindlimbs were processed for SEM-EDX and subsequently for immunofluorescence staining. Results: The localization of calcium, phosphorus, and carbon with type II collagen could be visualized in the same region of calcified growth plate cartilage on the same section. Conclusions: The combination of SEM-EDX and immunostaining is effective for visualizing mineral and protein distribution in the same histological sections.

本文言語	English
ページ（範囲）	461-464
ページ数	4
ジャーナル	journal of oral biosciences
巻	64
号	4
DOI	https://doi.org/10.1016/j.job.2022.08.005
出版ステータス	Published - 2022 12月

ASJC Scopus subject areas

医学（その他）
歯科学（全般）
生化学、遺伝学、分子生物学（全般）

文献へのアクセス

10.1016/j.job.2022.08.005

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引用スタイル

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title = "The visualization of the mineral and protein distribution in the same histological sections of rat calcified growth plate cartilage",

abstract = "Objective: To determine whether the combination of scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDX) and immunostaining would visualize the mineral and protein distribution in the same histological sections. Methods: Paraffin sections of fixed rat hindlimbs were processed for SEM-EDX and subsequently for immunofluorescence staining. Results: The localization of calcium, phosphorus, and carbon with type II collagen could be visualized in the same region of calcified growth plate cartilage on the same section. Conclusions: The combination of SEM-EDX and immunostaining is effective for visualizing mineral and protein distribution in the same histological sections.",

keywords = "Calcification, Cartilage, Immunofluorescence staining, SEM-EDX, Type II collagen",

author = "Miyuki Mayanagi and Megumi Nakamura and Akiko Henmi and Yasuyuki Sasano",

note = "Funding Information: We thank Mr. Yasuto Mikami for his helpful technical assistance. This work was supported by JSPS KAKENHI grant numbers JP17K17081 , 18K09517 , JP18K09760 , 21K09825 , and 21K10061 . Publisher Copyright: {\textcopyright} 2022 Japanese Association for Oral Biology",

year = "2022",

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doi = "10.1016/j.job.2022.08.005",

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TY - JOUR

T1 - The visualization of the mineral and protein distribution in the same histological sections of rat calcified growth plate cartilage

AU - Mayanagi, Miyuki

AU - Nakamura, Megumi

AU - Henmi, Akiko

AU - Sasano, Yasuyuki

N1 - Funding Information: We thank Mr. Yasuto Mikami for his helpful technical assistance. This work was supported by JSPS KAKENHI grant numbers JP17K17081 , 18K09517 , JP18K09760 , 21K09825 , and 21K10061 . Publisher Copyright: © 2022 Japanese Association for Oral Biology

PY - 2022/12

Y1 - 2022/12

N2 - Objective: To determine whether the combination of scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDX) and immunostaining would visualize the mineral and protein distribution in the same histological sections. Methods: Paraffin sections of fixed rat hindlimbs were processed for SEM-EDX and subsequently for immunofluorescence staining. Results: The localization of calcium, phosphorus, and carbon with type II collagen could be visualized in the same region of calcified growth plate cartilage on the same section. Conclusions: The combination of SEM-EDX and immunostaining is effective for visualizing mineral and protein distribution in the same histological sections.

AB - Objective: To determine whether the combination of scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDX) and immunostaining would visualize the mineral and protein distribution in the same histological sections. Methods: Paraffin sections of fixed rat hindlimbs were processed for SEM-EDX and subsequently for immunofluorescence staining. Results: The localization of calcium, phosphorus, and carbon with type II collagen could be visualized in the same region of calcified growth plate cartilage on the same section. Conclusions: The combination of SEM-EDX and immunostaining is effective for visualizing mineral and protein distribution in the same histological sections.

KW - Calcification

KW - Cartilage

KW - Immunofluorescence staining

KW - SEM-EDX

KW - Type II collagen

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