The usefulness of the decellularized matrix from three-dimensional regenerative cartilage as a scaffold material

Tomohiko Watanabe, Yukiyo Asawa, Makoto Watanabe, Ryuji Okubo, Masaki Nio, Tsuyoshi Takato, Kazuto Hoshi, Atsuhiko Hikita

研究成果: Article査読

抄録

In cartilage tissue engineering, research on materials for three-dimensional (3D) scaffold has attracted attention. Decellularized matrix can be one of the candidates for the scaffold material. In this study, decellularization of regenerated cartilage was carried out and its effectiveness as a scaffold material was examined. Three-dimensionally-cultured cartilage constructs in the differentiation medium containing IGF-1 produced more cartilage matrix than those in the proliferation medium. Detergent-enzymatic method (DEM) could decellularize 3D-cultured cartilage constructs only by 1 cycle without breaking down the structure of the constructs. In vitro, newly-seeded chondrocytes were infiltrated and engrafted into decellularized constructs in the proliferation medium, and newly formed fibers were observed around the surface where newly-seeded cells were attached. Recellularized constructs could mature similarly as those without decellularization in vivo. The decellularized 3D-cultured matrix from regenerative cartilage is expected to be used as a scaffold material in the future.

本文言語English
ページ(範囲)312-322
ページ数11
ジャーナルRegenerative Therapy
15
DOI
出版ステータスPublished - 2020 12

ASJC Scopus subject areas

  • 生体材料
  • 生体医工学
  • 発生生物学

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