The use of species-specific DNA markers for assessing alien chromosome transfer in Brassica rapa and Brassica oleracea-monosomic additions of Raphanus sativus

Kaoru Tonosaki, Michiko Akaba, Sang Woo Bang, Hiroyasu Kitashiba, Yukio Kaneko, Takeshi Nishio

研究成果: Article査読

5 被引用数 (Scopus)

抄録

Monosomic addition lines (MALs) are useful materials not only for cytogenetic and molecular genetic studies but also for plant breeding as gene sources. In our previous study, two MALs in the tribe Brassiceae were developed, one being Raphanus sativus lines with alien chromosomes of Brassica rapa (B. rapa-monosomic addition lines; BrMALs) and the second being those with alien chromosomes of Brassica oleracea (B. oleracea-monosomic addition lines; BoMALs). We developed species-specific DNA markers from the genomic sequences of B. rapa and B. oleracea comparing them with those of R. sativus, and identified chromosomes added in BrMALs and BoMALs using these markers. It was revealed that eight types of BrMALs have seven chromosomes of B. rapa and seven types of BoMALs have six chromosomes of B. oleracea. Furthermore, chromosome breakage and homoeologous recombination were suggested to have occurred in some MALs. The developed species-specific DNA markers are considered to be useful for producing MALs and also for assessing chromosome abnormality in MALs.

本文言語English
ページ(範囲)1301-1311
ページ数11
ジャーナルMolecular Breeding
34
3
DOI
出版ステータスPublished - 2014 10

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分子生物学
  • 農業および作物学
  • 遺伝学
  • 植物科学

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