The structural characteristics and the functional evaluation of nanogel

Wakiko Asayama, Masaki Takagi, Shin Ichi Sawada, Nobuyuki Morimoto, Tadashi Narita, Kazunari Akiyoshi

研究成果: Paper査読

抄録

GroEL or nanogel-mediated folding of β-D-galactosidase was examined. Urea-denatured β-D-galactosidase can spontaneously fold upon the dilution into the low urea concentration (about 15 %). In the presence of GroEL or ALP nanogel, folding of β-D-galactosidase was started by following the dilution. About 50 % of β-D-galactosidase recovered to native structure in both systems of GroEL and dodecyl group-bearing pullulan (ALP) nanogel recovered to native structure. The addition of ATP for GroEL has little effect on refolding. In contrast, the folding was arrested in the presence of CHP nanogels. Even by the addition of methyl-β-cyclodextrin for CHP nanogels, only 15 % of β-D-galactosidase activity recovered. Hydrophobie groups in the nanogels significantly affect chaperon-like activity.

本文言語English
ページ数1
出版ステータスPublished - 2005 12 1
外部発表はい
イベント54th SPSJ Annual Meeting 2005 - Yokohama, Japan
継続期間: 2005 5 252005 5 27

Other

Other54th SPSJ Annual Meeting 2005
国/地域Japan
CityYokohama
Period05/5/2505/5/27

ASJC Scopus subject areas

  • 工学(全般)

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