The role of angiogenesis in the tumor growth of Syrian hamster pancreatic cancer cell line HPD-NR

Shin Ichi Egawa, Masahiro Tsutsumi, Yoichi Konishi, Masao Kobari, Seiki Matsuno, Koichi Nagasaki, Hitoyasu Futami, Ken Yamaguchi

研究成果: Article査読

36 被引用数 (Scopus)

抄録

Background/Aims New therapeutic approach is required for pancreatic cancer, one of the most intractable malignancies. The role of angiogenesis in the tumor growth of a Syrian hamster pancreatic cancer cell line HPD-NR, which closely resembles its human counterpart, was investigated. Methods Angiogenic activity was measured as stimulation of growth of human umbilical vein endothelial cells (HUVEC), and angiogenic factors produced by HPD-NR cells were identified by reverse-transcription polymerase chain reaction and Northern blot analysis. Then in vitro and in vivo antitumor effects of a potent angiogenesis inhibitor, O-(chloroacetylcarbamoyl)fumagillol (AGM-1470), were examined. Results The conditioned medium of HPD-NR cells stimulated the growth of HUVEC, and four hamster angiogenic factors were detected with an overexpression of transforming growth factor α and vascular endothelial growth factor messenger RNAs. AGM-1470 specifically inhibited the growth of HUVEC and that of HPD-NR tumors in vivo with decreased vascularity of the tumors but not the growth of HPD-NR cells in vitro. Conclusions The results suggest that angiogenesis plays an important role in tumor growth of HPD-NR cells and can be a new target of medical therapy for pancreatic cancer.

本文言語English
ページ(範囲)1526-1533
ページ数8
ジャーナルGastroenterology
108
5
DOI
出版ステータスPublished - 1995 5
外部発表はい

ASJC Scopus subject areas

  • 肝臓学
  • 消化器病学

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