The regulation of amiloride-sensitive epithelial sodium channels by tumor necrosis factor-alpha in injured lungs and alveolar type II cells

Toshiyuki Yamagata, Yuko Yamagata, Takeshi Nishimoto, Tsunahiko Hirano, Masanori Nakanishi, Yoshiaki Minakata, Masakazu Ichinose, André Dagenais, Yves Berthiaume

研究成果: Article査読

29 被引用数 (Scopus)

抄録

Alveolar liquid clearance, which mainly depends on sodium transport in alveolar epithelial cells, is an important mechanism by which excess water in the alveoli is reabsorbed during the resolution of pulmonary edema. In this study, we examined the regulation of epithelial sodium channel (ENaC), the main contributor to sodium transport, during acute lung injury and the direct impact of tumor necrosis factor-alpha (TNF-α), one of the important cytokines in acute lung injury, on the ENaC regulation. During the development of pulmonary edema, the increases in the number of neutrophils and the levels of TNF-α in blood and bronchoalveolar lavage were seen. In parallel, the mRNA expression of the α-, β- and γ-ENaC subunits in the whole lung tissue was inhibited to 72.0, 47.8 and 53.9%, respectively. The direct exposure of rat alveolar type II cells to TNF-α inhibited the mRNA expression of α- and γ-ENaC to 64.0 and 78.0%, but not that of the β-ENaC. TNF-α also inhibited the ENaC function as indicated by the reduction of amiloride-sensitive current (control 4.4, TNF-α 1.9 μA/cm2). These data suggest that TNF-α may affect the pathophysiology of acute lung injury and pulmonary edema through the inhibition of alveolar liquid clearance and sodium transport.

本文言語English
ページ(範囲)16-23
ページ数8
ジャーナルRespiratory Physiology and Neurobiology
166
1
DOI
出版ステータスPublished - 2009 3 31
外部発表はい

ASJC Scopus subject areas

  • 神経科学(全般)
  • 生理学
  • 呼吸器内科

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